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human nlrp3 mcherry constructs  (Addgene inc)


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    Structured Review

    Addgene inc human nlrp3 mcherry constructs
    A. Schematic representation of human NLRP6 and <t>NLRP3</t> showing their domain structures and the respective polybasic regions within the FISNA of mouse and human NLRP3 and 6 aligned. B. Flow cytometry-based quantification of ASC speck formation in GFP-ASC tg HEK293T cells expressing hNLRP6-mCherry or hNLRP3-mCherry infected with WT or τ< hly L. monocytogenes EGD for 6h, treated with nigericin for 1h or with imiquimod for 6h in presence or absence of 60 mM extracellular KCl. C. Flow cytometry based quantification of ASC speck formation in GFP-ASC tg HEK293T cells, expressing the indicated NLRP6 proteins, infected with WT or τ< hly L. monocytogenes EGD for 6h D. Flow cytometry based quantification of ASC speck formation in GFP-ASC tg HEK293T cells expressing either NLRP6-WT, NLRP3-WT or the indicated chimeric NLRP6-3 proteins with mCherry tags, infected with WT or τ< hly L. monocytogenes EGD for 6h, treated with nigericin for 1h or with imiquimod for 6h. E. Flow cytometry based quantification of ASC speck formation in GFP-ASC tg HEK293T cells, expressing NLRP6 τιLRR -mCherry, infected with WT or τ< hly L. monocytogenes EGD for 6h. F. Flow cytometry based quantification of ASC speck formation in GFP-ASC tg HEK293T cells, expressing NLRP6 WA/WB , infected with WT or τ< hly L. monocytogenes EGD for 6h. Graphs show mean ± SD from three pooled experiments. ns = non-significant, **P < 0.01; ***P < 0.001, ****P < 0.0001 (two-way ANOVA with either Tukey’s multiple comparisons test (B), Dunett’s multiple comparisons test (C,E) or Uncorrected Fisher’s LSD (F)).
    Human Nlrp3 Mcherry Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human nlrp3 mcherry constructs/product/Addgene inc
    Average 93 stars, based on 27 article reviews
    human nlrp3 mcherry constructs - by Bioz Stars, 2026-03
    93/100 stars

    Images

    1) Product Images from "Sterile- or pathogen-induced endolysosomal damage activate the NLRP6 inflammasome in human intestinal epithelial cells"

    Article Title: Sterile- or pathogen-induced endolysosomal damage activate the NLRP6 inflammasome in human intestinal epithelial cells

    Journal: bioRxiv

    doi: 10.1101/2025.01.23.634286

    A. Schematic representation of human NLRP6 and NLRP3 showing their domain structures and the respective polybasic regions within the FISNA of mouse and human NLRP3 and 6 aligned. B. Flow cytometry-based quantification of ASC speck formation in GFP-ASC tg HEK293T cells expressing hNLRP6-mCherry or hNLRP3-mCherry infected with WT or τ< hly L. monocytogenes EGD for 6h, treated with nigericin for 1h or with imiquimod for 6h in presence or absence of 60 mM extracellular KCl. C. Flow cytometry based quantification of ASC speck formation in GFP-ASC tg HEK293T cells, expressing the indicated NLRP6 proteins, infected with WT or τ< hly L. monocytogenes EGD for 6h D. Flow cytometry based quantification of ASC speck formation in GFP-ASC tg HEK293T cells expressing either NLRP6-WT, NLRP3-WT or the indicated chimeric NLRP6-3 proteins with mCherry tags, infected with WT or τ< hly L. monocytogenes EGD for 6h, treated with nigericin for 1h or with imiquimod for 6h. E. Flow cytometry based quantification of ASC speck formation in GFP-ASC tg HEK293T cells, expressing NLRP6 τιLRR -mCherry, infected with WT or τ< hly L. monocytogenes EGD for 6h. F. Flow cytometry based quantification of ASC speck formation in GFP-ASC tg HEK293T cells, expressing NLRP6 WA/WB , infected with WT or τ< hly L. monocytogenes EGD for 6h. Graphs show mean ± SD from three pooled experiments. ns = non-significant, **P < 0.01; ***P < 0.001, ****P < 0.0001 (two-way ANOVA with either Tukey’s multiple comparisons test (B), Dunett’s multiple comparisons test (C,E) or Uncorrected Fisher’s LSD (F)).
    Figure Legend Snippet: A. Schematic representation of human NLRP6 and NLRP3 showing their domain structures and the respective polybasic regions within the FISNA of mouse and human NLRP3 and 6 aligned. B. Flow cytometry-based quantification of ASC speck formation in GFP-ASC tg HEK293T cells expressing hNLRP6-mCherry or hNLRP3-mCherry infected with WT or τ< hly L. monocytogenes EGD for 6h, treated with nigericin for 1h or with imiquimod for 6h in presence or absence of 60 mM extracellular KCl. C. Flow cytometry based quantification of ASC speck formation in GFP-ASC tg HEK293T cells, expressing the indicated NLRP6 proteins, infected with WT or τ< hly L. monocytogenes EGD for 6h D. Flow cytometry based quantification of ASC speck formation in GFP-ASC tg HEK293T cells expressing either NLRP6-WT, NLRP3-WT or the indicated chimeric NLRP6-3 proteins with mCherry tags, infected with WT or τ< hly L. monocytogenes EGD for 6h, treated with nigericin for 1h or with imiquimod for 6h. E. Flow cytometry based quantification of ASC speck formation in GFP-ASC tg HEK293T cells, expressing NLRP6 τιLRR -mCherry, infected with WT or τ< hly L. monocytogenes EGD for 6h. F. Flow cytometry based quantification of ASC speck formation in GFP-ASC tg HEK293T cells, expressing NLRP6 WA/WB , infected with WT or τ< hly L. monocytogenes EGD for 6h. Graphs show mean ± SD from three pooled experiments. ns = non-significant, **P < 0.01; ***P < 0.001, ****P < 0.0001 (two-way ANOVA with either Tukey’s multiple comparisons test (B), Dunett’s multiple comparisons test (C,E) or Uncorrected Fisher’s LSD (F)).

    Techniques Used: Flow Cytometry, Expressing, Infection



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    human nlrp3 mcherry constructs  (Addgene inc)
    93
    Addgene inc human nlrp3 mcherry constructs
    A. Schematic representation of human NLRP6 and <t>NLRP3</t> showing their domain structures and the respective polybasic regions within the FISNA of mouse and human NLRP3 and 6 aligned. B. Flow cytometry-based quantification of ASC speck formation in GFP-ASC tg HEK293T cells expressing hNLRP6-mCherry or hNLRP3-mCherry infected with WT or τ< hly L. monocytogenes EGD for 6h, treated with nigericin for 1h or with imiquimod for 6h in presence or absence of 60 mM extracellular KCl. C. Flow cytometry based quantification of ASC speck formation in GFP-ASC tg HEK293T cells, expressing the indicated NLRP6 proteins, infected with WT or τ< hly L. monocytogenes EGD for 6h D. Flow cytometry based quantification of ASC speck formation in GFP-ASC tg HEK293T cells expressing either NLRP6-WT, NLRP3-WT or the indicated chimeric NLRP6-3 proteins with mCherry tags, infected with WT or τ< hly L. monocytogenes EGD for 6h, treated with nigericin for 1h or with imiquimod for 6h. E. Flow cytometry based quantification of ASC speck formation in GFP-ASC tg HEK293T cells, expressing NLRP6 τιLRR -mCherry, infected with WT or τ< hly L. monocytogenes EGD for 6h. F. Flow cytometry based quantification of ASC speck formation in GFP-ASC tg HEK293T cells, expressing NLRP6 WA/WB , infected with WT or τ< hly L. monocytogenes EGD for 6h. Graphs show mean ± SD from three pooled experiments. ns = non-significant, **P < 0.01; ***P < 0.001, ****P < 0.0001 (two-way ANOVA with either Tukey’s multiple comparisons test (B), Dunett’s multiple comparisons test (C,E) or Uncorrected Fisher’s LSD (F)).
    Human Nlrp3 Mcherry Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human nlrp3 mcherry constructs/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    human nlrp3 mcherry constructs - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    A. Schematic representation of human NLRP6 and NLRP3 showing their domain structures and the respective polybasic regions within the FISNA of mouse and human NLRP3 and 6 aligned. B. Flow cytometry-based quantification of ASC speck formation in GFP-ASC tg HEK293T cells expressing hNLRP6-mCherry or hNLRP3-mCherry infected with WT or τ< hly L. monocytogenes EGD for 6h, treated with nigericin for 1h or with imiquimod for 6h in presence or absence of 60 mM extracellular KCl. C. Flow cytometry based quantification of ASC speck formation in GFP-ASC tg HEK293T cells, expressing the indicated NLRP6 proteins, infected with WT or τ< hly L. monocytogenes EGD for 6h D. Flow cytometry based quantification of ASC speck formation in GFP-ASC tg HEK293T cells expressing either NLRP6-WT, NLRP3-WT or the indicated chimeric NLRP6-3 proteins with mCherry tags, infected with WT or τ< hly L. monocytogenes EGD for 6h, treated with nigericin for 1h or with imiquimod for 6h. E. Flow cytometry based quantification of ASC speck formation in GFP-ASC tg HEK293T cells, expressing NLRP6 τιLRR -mCherry, infected with WT or τ< hly L. monocytogenes EGD for 6h. F. Flow cytometry based quantification of ASC speck formation in GFP-ASC tg HEK293T cells, expressing NLRP6 WA/WB , infected with WT or τ< hly L. monocytogenes EGD for 6h. Graphs show mean ± SD from three pooled experiments. ns = non-significant, **P < 0.01; ***P < 0.001, ****P < 0.0001 (two-way ANOVA with either Tukey’s multiple comparisons test (B), Dunett’s multiple comparisons test (C,E) or Uncorrected Fisher’s LSD (F)).

    Journal: bioRxiv

    Article Title: Sterile- or pathogen-induced endolysosomal damage activate the NLRP6 inflammasome in human intestinal epithelial cells

    doi: 10.1101/2025.01.23.634286

    Figure Lengend Snippet: A. Schematic representation of human NLRP6 and NLRP3 showing their domain structures and the respective polybasic regions within the FISNA of mouse and human NLRP3 and 6 aligned. B. Flow cytometry-based quantification of ASC speck formation in GFP-ASC tg HEK293T cells expressing hNLRP6-mCherry or hNLRP3-mCherry infected with WT or τ< hly L. monocytogenes EGD for 6h, treated with nigericin for 1h or with imiquimod for 6h in presence or absence of 60 mM extracellular KCl. C. Flow cytometry based quantification of ASC speck formation in GFP-ASC tg HEK293T cells, expressing the indicated NLRP6 proteins, infected with WT or τ< hly L. monocytogenes EGD for 6h D. Flow cytometry based quantification of ASC speck formation in GFP-ASC tg HEK293T cells expressing either NLRP6-WT, NLRP3-WT or the indicated chimeric NLRP6-3 proteins with mCherry tags, infected with WT or τ< hly L. monocytogenes EGD for 6h, treated with nigericin for 1h or with imiquimod for 6h. E. Flow cytometry based quantification of ASC speck formation in GFP-ASC tg HEK293T cells, expressing NLRP6 τιLRR -mCherry, infected with WT or τ< hly L. monocytogenes EGD for 6h. F. Flow cytometry based quantification of ASC speck formation in GFP-ASC tg HEK293T cells, expressing NLRP6 WA/WB , infected with WT or τ< hly L. monocytogenes EGD for 6h. Graphs show mean ± SD from three pooled experiments. ns = non-significant, **P < 0.01; ***P < 0.001, ****P < 0.0001 (two-way ANOVA with either Tukey’s multiple comparisons test (B), Dunett’s multiple comparisons test (C,E) or Uncorrected Fisher’s LSD (F)).

    Article Snippet: Human NLRP3-mCherry constructs are derived from a pEGFP-C2-NLRP3 plasmid (addgene #73955), while human NLRP6-mCherry constructs were derived from the plasmid mentioned above.

    Techniques: Flow Cytometry, Expressing, Infection